Bioinformatics MCQ

	1.If you were using a proteomics approach to find the cause of a muscle disorder, which of the following techniques might you be using? creating a genomic library sequencing the gene responsible for the disorder developing physical maps from genomic clones determining which environmental factors influence the expression of your gene of interest annotating the gene sequence

	2.Shotgun cloning differs from the clone-by-clone method in which of the following ways? The location of the clone being sequenced is known relative to other clones within the genomic library in shotgun cloning. Genetic markers are used to identify clones in shotgun cloning. Computer software assembles the clones in the clone-by-clone method. The entire genome is sequenced in the clone-by-clone method, but not in shotgun sequencing. No genetic or physical maps of the genome are needed to begin shotgun cloning.

	3.CpG islands and codon bias are tools used in eukaryotic genomics to __________. identify open reading frames differentiate between eukaryotic and prokaryotic DNA sequences find regulatory sequences look for DNA-binding domains identify a gene’s function

	4.As the complexity of an organism increases, all of the following characteristics emerge except __________. the gene density decreases the number of introns increases the gene size increases an increase in the number of chromosomes repetitive sequences are present

	5.Gene duplication has been found to be one of the major reasons for genome expansion in eukaryotes. In general, what would be the selective advantage of gene duplication? If one gene copy is nonfunctional, a backup is available. Larger genomes are more resistant to spontaneous mutations. Duplicated genes will make more of the protein product. Gene duplication will lead to new species evolution.

	6.How are so many different antibodies produced from fewer than 300 major genes? gene duplication alternative splicing mechanisms the formation of polyproteins the formation of nonspecific B cells recombination, deletions, and random assortment of DNA segments

	7.Two-dimensional gels are used to __________. separate DNA fragments separate RNA fragments separate different proteins observe a protein in two dimensions separate DNA from RNA

	8.What would be a likely explanation for the existence of pseudogenes? gene duplication gene duplication and mutation events mutation events unequal crossing over evolutionary pressure

http://wps.prenhall.com/esm_klug_essentials_5/17/4576/1171660.cw/-/1171662/index.html

1. The length of each coil in DNA strand is

	30 Ao
	5 Ao
	34 Ao
	15 Ao

2. The diagrammatic representation of the total no. of genes in DNA is

	Gene map
	Genome
	Gene-structure
	Chromatin

3. The best studied example for specialized transduction is

	P1 phage
	Both a and c
	P22 phage
	ë-phage

4. The bases Adenine and Thymine are paired with

	Single hydrogen bonds
	Both b and c
	Triple hydrogen bonds
	Double hydrogen bonds

5. During specialized transduction

	Large amound of DNA is transferred
	A few no. of genes are transferred
	None of these
	Whole DNA is transferred

6. Nucleic acids are highly charged polymers due to

	Nucleotides are charged structures
	They have positive and negative ends
	Nitrogenous bases are highly ionized compounds
	There is phosphodiester bond between 5’- hydroxyl of one ribose and 3’–hydroxyl of next ribose

7. The no. of hydrogen bonds existing between Guanine and Cytosine are

	3
	2
	None of these
	5

8. Genetic information transfer DNA to RNA is called –

	Transcriptase
	Transduction
	Transformation
	Recombination

9. The gene transfer occurs by –

	Transformation
	Transduction
	Cell fusion
	Conjugation

10. The cell donating DNA during transformation is

	Endogenate
	Mesozygote
	Merosite
	Exogenate

http://mcq.4istudent.com/nucleic-acids-genetics-biotechnology-bioinformatics-zoology-botany-free-multiple-choice-quiz/